Abbas Jafarian‐Dehkordi

Isfahan University of Medical Sciences

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Mahmoud Etebari

Isfahan University of Medical Sciences

Mohammad Rabbani

Isfahan University of Medical Sciences

Behzad Zolfaghari

Isfahan University of Medical Sciences

M. Hamid

Kerman University of Medical Sciences

Hojjat Sadeghi‐Aliabadi

Isfahan University of Medical Sciences

Seyed Ebrahim Sajjadi

Isfahan University of Medical Sciences

Jaleh Varshosaz

Isfahan University of Medical Sciences

Leila Safaeian

Isfahan University of Medical Sciences

Seyed Ahmad Emami

Mashhad University of Medical Sciences

Alireza Ghannadi

Fasa University of Medical Sciences

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Isfahan University of Medical Sciences

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University of Isfahan

Tehran University of Medical Sciences

Shahid Beheshti University of Medical Sciences

Pharmaceutical Sciences Research Center

University of Tehran

Academic Center for Education, Culture and Research

Tarbiat Modares University

Royan Institute

Isfahan University of Technology

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THE EFFECTS OF SALVIA HYDRANGEA ON CELL-MEDIATED IMMUNE RESPONSE IN MICE

Abbas Jafarian‐Dehkordi Alireza Ghannadi Nahid Keramati

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Phytochemical Screening and Cytotoxic Evaluation of Euphorbia turcomanica on Hela and HT-29 Tumor Cell Lines

Advanced Biomedical Research (2016)

Abbas Jafarian‐Dehkordi Mehdi Aliomrani Behzad Zolfaghari

Background: Cancer is a term for a large group of different diseases, all involving uncontrolled cell growth. Many of Euphorbiaceae plants have been traditionally used for the treatment of ulcers, tumors, warts, and other diseases. In addition, in the last decade, there are studies showing cytotoxic effects of different species of Euphorbia on tumor cell lines. In this study, we attempted to determine if Euphorbia turcomanica possess any cytotoxic activity. Materials and Methods: Solvents extracted the plant powder with various polarities by a maceration method, and qualitative phytochemical analyzes were carried out on them to identify the constituents. On the other hand, the possible cytotoxicity of different extracts on Hela and HT-29 tumor cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and 50% reduction in cell survival was considered as a cytotoxic effect. Analyze of variance followed by Student-Newman-Keuls test was used to see the differences among the groups. Results: Phytochemical analysis of E. turcomanica showed the presence of flavonoid, alkaloid, anthraquinone and tannin in plant aerial parts. Methanol-water, acetone, dichloromethane, methanol, and heptane extracts of E. turcomanica significantly reduced viability of Hela cells (P < 0.05) with inhibitory concentration 50% (IC50) of 50, 90, 230, 420, and 450 μg/ml, respectively. While methanol-water, dichloromethane, methanol, ethyl acetate, and heptane extracts were cytotoxic with IC50of 43, 115, 125, 250, and 390 μg/ml, respectively (P < 0.05), on HT-29 cells. Conclusion: It can be concluded that E. turcomanica is a good candidate for further study toward cytotoxic agents.

Phytochemical

HeLa

Euphorbia

Maceration (sewage)

Euphorbiaceae

Dichloromethane

Viability assay

10.4103/2277-9175.192734

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Citations (14)

Rapid quantitative determination of GSTP1 promoter methylation level using differential high resolution melting analysis (D-HRMA)

Research in Pharmaceutical Sciences (2012)

Mitra Torabi Javad Mohammadi‐Asl M. Hamid Mohammad Rabbani Abbas Jafarian‐Dehkordi

Background and Aims: DNA methylation is one of the key regulators of gene expression. It also has an important role in carcinogenesis through epigenetic silencing of tumor suppressor and DNA repair genes. DNA hyper methylation in promoter region of glutathione S-transferase pi 1 (GSTP1) gene has been detected in a variety of cancers such as breast cancer. As studies show, GSTP1 promoter methylation status can be used as a prognostic and predictive factor in breast cancer. This study was designed to quantitatively determine the promoter methylation of GSTP1 gene in archival formalin fixed paraffin embedded (FFPE) tissues from breast cancer patients by differential high resolution melting analysis (D -HRMA) method. Methods: Using D-HRMA technology, the methylation level of GSTP1 gene promoter was quantified in 98 breast cancer FFPE tissues and also 10 fresh frozen normal tissue samples. Results: All the breast cancer samples were found to be methylated at the GSTP1 promoter region, with the average methylation level of 41.56% (range 3.93- 66.61%). The methylation level of normal samples was identified below 1%. Statistical analyses showed a significant correlation between the GSTP1 promoter methylation levels and cancer progression (P< 0.001). No significant correlation was observed between the methylation levels and patients’ age and gender. Conclusions: It is concluded that GSTP1 promoter methylation level can be used as a reliable and sensitive diagnostic and prognostic tool in breast cancer. Also D -HRMA is demonstrated as a robust, fast and cost effective method for quantitative evaluation of promoter methylation.

GSTP1

High Resolution Melt

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COMPARISON OF ACUTE AND CHRONIC EFFECTS OF NIFEDIPINE ON NALOXONE-PRECIPITATED MORPHINE WITHDRAWAL IN MICE

The Medical Journal of The Islamic Republic of Iran (2003)

Mohammad Rabbani Abbas Jafarian‐Dehkordi Mh Sobhanian

Medical journal

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PHYSICOCHEMICAL AND CLINICAL EVALUATION OF CALCIUM ACETATE TABLETS

Naser Tavakoli Abbas Jafarian‐Dehkordi Sheila Shahidi Mohammad Hadi Dehghani

Phosphorous binders such as calcium carbonate (CC) and aluminum hydroxide have been used in Chronic Renal Failure (CRF) patients to decrease hyperphosphatemia. The aim of this study was to prepare calcium acetate (CA) tablets and evaluate their effects in CRF patients. CA tablets were prepared by wet granulation method and their physicochemical properties such as weight variation, hardness, friability, disintegration time and dissolution rate were studied. Calcium was determined by atomic absorption method. The in vitro phosphorus binding capacity of CA was evaluated and compared with CC tablets marketed in Iran. The stability of prepared tablets was studied as well. Clinical usefulness of CA as phosphorus binder was assessed in 24 adult patients. Mean weight, hardness and disintegration time of CA tablets were 1161.5 mg, 49.5 N and 34.5 min, respectively. Percent of the drug released after 30 and 60 minutes were 76.2% and 97.6%, respectively. The tablets released 50% of their content up to 18.4 min. Percent of phosphorus insolublized as calcium phosphate by CC and CA were 30.7% and 16.2% respectively. CA tablets disintegration time in acidic, neutral and basic media were 39.5, 34.4 and 42.2 min. Physicochemical properties of tablets during the stability test condition were acceptable based on FDA standards. CA decreased serum phosphorus concentration more effectively than CC. Our data confirm that a given dose of calcium administrated orally as CA, binds more phosphorus in the intestine than the same dose of CC. This may be due to the better solubility of CA.

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Granulation

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Effects ofRS-albuterol on the development of antigen-mediated airway hyperreactivity in guinea pigs

Clinical Reviews in Allergy & Immunology (1996)

Abbas Jafarian‐Dehkordi Dean A. Handley D F Biggs

10.1007/bf02772205

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Citations (15)

Role of recombinant human erythropoietin loading chitosan-tripolyphosphate nanoparticles in busulfan-induced genotoxicity: Analysis of DNA fragmentation via comet assay in cultured HepG2 cells

Toxicology in Vitro (2016)

Nasrin Ghassemi-Barghi Jaleh Varshosaz Mahmoud Etebari Abbas Jafarian‐Dehkordi

Comet Assay

Fragmentation

10.1016/j.tiv.2016.07.001

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Effect of preoperative systemic capsaicin on tooth sensitivity after in-office bleaching: A pilot study

Dental Research Journal (2024)

Pouran Samimi Marzie Kachuie Abbas Jafarian‐Dehkordi Maryam Montazeri Shahtouri Maryam Khoroushi

Background: Tooth bleaching sensitivity (TBS) after bleaching procedures is a common problem. This study was undertaken to determine the effect of preoperative systemic capsaicin on tooth sensitivity (TS) after in-office bleaching procedures. Materials and Methods: Thirty participants received the treatment in this clinical trial. The subjects were randomly assigned to two groups ( n = 15). Placebo and 0.25% capsaicin were administered three times daily for 24 h, with the first dose being administrated 1 h before the bleaching procedure. The subjects underwent two bleaching sessions at a 2-week interval by applying 40% hydrogen peroxide gel on six upper anterior teeth. A visual analog scale (VAS) was used to evaluate TS. Data were analyzed with SPSS 24. Statistical analyses were carried out with the Wilcoxon test and paired t -test. Statistical significance was set at P ≤ 0.05. Results: In the capsaicin group, there was a significant increase in TBS between the immediate and 1-h postoperative intervals and a significant decrease between 1- and 24-h postoperative intervals ( P = 0.01 and P = 0.000, respectively). In the placebo group, there was a significant decrease between immediate and 24-h and between 1- and 24-h postoperative intervals ( P = 0.007, P = 0.02). Milder TS was detected in the placebo group 24 h after bleaching ( P < 0.05). Conclusion: Under the limitations of this study, preoperative use of systemic capsaicin did not significantly affect TS after the in-office bleaching procedure.

Capsaicin

Tooth Sensitivity

Statistical Analysis

10.4103/drj.drj_326_23

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Quantitation of CDH1 promoter methylation in formalin-fixed paraffin-embedded tissues of breast cancer patients using differential high resolution melting analysis

Advanced Biomedical Research (2016)

HamidMir Mohammad Sadeghi Mojgan Naghitorabi Javad Mohammadi‐Asl Mohammad Rabbani Abbas Jafarian‐Dehkordi

Background: E-cadherin (CDH1) plays an important role in cell–cell adhesion of epithelial tissues. Loss of E-cadherin expression can lead to loss of tissue integrity, metastasis, and cancer progression. Also loss of E-cadherin expression might be related to aberrant promoter methylation of the CDH1 gene. Many studies have been performed on CDH1 promoter methylation, especially in breast cancer. Although most of the studies have used qualitative methods for methylation analysis, this study is designed to quantitatively investigate CDH1 promoter methylation in breast cancer and its correlation with patients' clinicopathological features. Materials and Methods: Using differential high resolution melting analysis (D-HRMA), the methylation level of the CDH1 gene promoter was quantified in 98 breast cancer formalin-fixed paraffin-embedded (FFPE) tissues and also 10 fresh frozen normal breast tissues. Results: All samples were detected to be methylated at the CDH1 promoter region. About 74.5% of the breast cancer samples were hypermethylated with an average methylation level of around 60%, while 25.5% of the patients were methylated with the mean methylation level of about 33%, and 90% of the normal samples had a mean methylation level of about 18%. Statistical analyses represented a significant correlation between CDH1 promoter methylation and cancer progression hallmarks, such as, clinical stage, nodal involvement, tumor size, and histological grade. Conclusion: In summary, quantitation of CDH1 promoter methylation can serve as a diagnostic and prognostic tool in breast cancer. Also D-HRMA can be used as a fast and reliable method for quantitation of promoter methylation.

CDH1

High Resolution Melt

10.4103/2277-9175.183139

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Citations (6)

Anxiolytic effects of Salvia reuterana Boiss. on the elevated plus-maze model of anxiety in mice

Journal of Ethnopharmacology (2005)

Mohammad Rabbani Seyed Ebrahim Sajjadi Abbas Jafarian‐Dehkordi Golnaz Vaseghi

Elevated plus maze

Salvia

Locomotor activity

10.1016/j.jep.2005.03.034

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Citations (48)