Yu‐Ting Tsao

Chang Gung Memorial Hospital

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Chao‐Min Cheng

National Tsing Hua University

Hung‐Chi Chen

Chang Gung Memorial Hospital

Yi‐Jen Hsueh

Chang Gung Memorial Hospital

Wei‐Chi Wu

Chang Gung Memorial Hospital

Chung‐Yao Yang

Hygeia Hospital

Kuan‐Jen Chen

Chang Gung University

Koji Matsuura

Okayama University of Science

Yih‐Shiou Hwang

Chang Gung University

Ting‐Chang Chang

Chang Gung Memorial Hospital

Po‐Han Yeh

Chang Gung Memorial Hospital

Cooperative Institutions

Chang Gung Memorial Hospital

Chang Gung University

National Tsing Hua University

Linkou Chang Gung Memorial Hospital

The University of Tokyo

Memorial Hospital of South Bend

University of Tsukuba

National Yang Ming Chiao Tung University

National Institute of Advanced Industrial Science and Technology

Japan Science and Technology Agency

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Point‐of‐care semen analysis of patients with infertility via smartphone and colorimetric paper‐based diagnostic device

Bioengineering & Translational Medicine (2020)

Yu‐Ting Tsao Chung‐Yao Yang Yun‐Chiao Wen Ting‐Chang Chang Koji Matsuura

Male infertility affects millions of males worldwide and is rising in prevalence due to social and environmental conditions. However, men often feel too embarrassed to receive a semen analysis in the hospital due to social stigmas. To overcome this problem, we developed a 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide test strip to distinguish semen samples with low total motile sperm concentration from those with normal motile sperm concentration. This is a point-of-care colorimetric semen analytical method with a one-step, inexpensive, equipment-free evaluation process, and adequate accuracy validated in a 42-sample clinical trial. In this study, results were evaluated visually and with a smartphone application. Using visual observation methods, the area under the curve (AUC) was 0.71 (95% of confidence interval = 0.55-0.86; p = 0.021), sensitivity was 41%, specificity was 95%, positive predictive value was 90%, negative predictive value (NPV) was 59.4%, and accuracy was 67%. Using a smartphone recording and analytical system, AUC was 0.766 (95% of confidence interval = 0.612-0.92; p = 0.003), sensitivity was 96%, specificity was 65%, PPV was 75%, NPV was 92.9%, and accuracy was 80.9%. This work demonstrated a screening tool that could elevate semen analysis to the level of routine healthcare and provide for private, in-home self-assessment.

Semen Analysis

Point of care

10.1002/btm2.10176

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Citations (25)

Author response for "Analysis of aqueous humor total antioxidant capacity and its correlation with corneal endothelial health"

Yu‐Ting Tsao Wei‐Chi Wu Kuan‐Jen Chen Lung‐Kun Yeh Yih‐Shiou Hwang

10.1002/btm2.10199/v2/response1

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Design, application, and integration of paper-based sensors with the Internet of Things

Elsevier eBooks (2019)

Jen-Hsuan Hsiao Yu‐Ting Tsao Chung‐Yao Yang Chao‐Min Cheng

Software portability

10.1016/b978-0-12-815409-0.00002-4

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Citations (1)

Protocol for assessing total antioxidant capacity in minimal volumes of varying clinical human samples

STAR Protocols (2024)

Yu‐Ting Tsao Yi‐Jen Hsueh Hung‐Chi Chen Chao‐Min Cheng

Total antioxidant capacity (TAC), representative of the capacity to combat oxidative stress, is closely linked to numerous diseases. Here, we present a protocol for measuring TAC using minimal samples that are stable across varying pH levels and at room temperature. We describe steps for preparing and loading samples and working solutions and conducting and analyzing the colorimetric reaction. Sample sources include aqueous humor, vitreous, tears, and plasma, which allow the protocol to be used in various clinical diagnostic settings. For complete details on the use and execution of this protocol, please refer to publications by Tsao et al. (2022).1,2

10.1016/j.xpro.2023.102822

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Author response for "Point‐of‐care semen analysis of patients with infertility via smartphone and colorimetric paper‐based diagnostic device"

Yu‐Ting Tsao Chung‐Yao Yang Yun‐Chiao Wen Ting‐Chang Chang Koji Matsuura

Point of care

Semen Analysis

10.1002/btm2.10176/v2/response1

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Impact of the COVID-19 Pandemic on Microbial Profiles and Clinical Outcomes in Orbital and Preseptal Cellulitis

Microorganisms (2024)

Yu‐Ting Tsao Yueh-Ju Tsai Chau-Yin Chen Yen-Chang Chu Yun-Shan Tsai

Orbital cellulitis and severe preseptal cellulitis are critical periocular infections with potential vision- and life-threatening implications. The COVID-19 pandemic is hypothesized to have had an influence on their presentation and pathogenesis; however, the real impact remains unclear. In this retrospective multicenter cohort study from January 2017 to December 2022, we analyzed 1285 cases with preseptal or orbital cellulitis in pre-pandemic (2017-2019) and pandemic (2020-2022) cohorts. A notable decrease in hospitalized cases during the pandemic period was observed (97 patients in the pre-pandemic group vs. 54 in the pandemic group,

Pandemic

2019-20 coronavirus outbreak

Orbital cellulitis

10.3390/microorganisms12112262

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Paper-Based ELISA: A Novel Diagnostic Approach for Monitoring Aqueous Humour VEGF Level in Ocular Diseases

IntechOpen eBooks (2019)

Yu‐Ting Tsao Cheyenne Wei‐Hsuan Sung Hung‐Chi Chen Min‐Yen Hsu Chao‐Min Cheng

We commonly diagnose ocular diseases via both morphological changes and symptoms. It is necessary to develop biochemically based assays for early or follow-up diagnosis of these diseases with a focus on robustness and ease of handling. To lay out a prospective path toward this goal, we describe and propose the use of ultrahigh sensitive paper-based ELISA (p-ELISA), which uses a treated piece of filter paper to monitor the activity of ocular diseases (i.e., detecting the vascular endothelial growth factor (VEGF) concentration in aqueous humour for proliferative diabetic retinopathy or age-related macular degeneration diagnosis). The advantages of p-ELISA include the following: (1) the capacity to directly measure biomarker concentrations in aqueous humour using only a tiny sample volume (as little as 2 μL); (2) significantly increased sensitivity compared to conventional ELISA (fg/mL levels); and (3) inexpensive materials and a short operation duration. P-ELISA is a novel point-of-care diagnostic tool with the significant potential to advance ophthalmological treatment guidelines by facilitating early detection and routinely monitoring therapeutic response.

Aqueous humour

10.5772/intechopen.81797

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List of Contributors

Elsevier eBooks (2019)

Takahiro Arakawa Hongmei Bi Chao‐Min Cheng Akihiro Chiba Shih-Wen Chiu

10.1016/b978-0-12-815409-0.00030-9

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The Pathomechanism, Antioxidant Biomarkers, and Treatment of Oxidative Stress-Related Eye Diseases

International Journal of Molecular Sciences (2022)

Yi‐Jen Hsueh Yen-Ning Chen Yu‐Ting Tsao Chao‐Min Cheng Wei‐Chi Wu

Oxidative stress is an important pathomechanism found in numerous ocular degenerative diseases. To provide a better understanding of the mechanism and treatment of oxidant/antioxidant imbalance-induced ocular diseases, this article summarizes and provides updates on the relevant research. We review the oxidative damage (e.g., lipid peroxidation, DNA lesions, autophagy, and apoptosis) that occurs in different areas of the eye (e.g., cornea, anterior chamber, lens, retina, and optic nerve). We then introduce the antioxidant mechanisms present in the eye, as well as the ocular diseases that occur as a result of antioxidant imbalances (e.g., keratoconus, cataracts, age-related macular degeneration, and glaucoma), the relevant antioxidant biomarkers, and the potential of predictive diagnostics. Finally, we discuss natural antioxidant therapies for oxidative stress-related ocular diseases.

10.3390/ijms23031255

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Citations (109)

An Assessment of Cataract Severity Based on Antioxidant Status and Ascorbic Acid Levels in Aqueous Humor

Antioxidants (2022)

Yu‐Ting Tsao Wei‐Chi Wu Kuan‐Jen Chen Chun‐Fu Liu Yi‐Jen Hsueh

Cataract is the leading cause of blindness throughout the world. Currently, the cataract severity evaluation is based on the subjective LOCS III guideline. To ameliorate the evaluation system and develop an objective and quantitative analysis, we investigated the relationships among aqueous humor total antioxidant capacity (AqTAC), ascorbic acid (AqAA) concentration, and cataract severity. In this study, we enrolled 130 cataract patients who underwent phacoemulsification between April 2019 and March 2020. The AqTAC and AqAA were measured by our own developed TAC assay and commercially available kit. Cataract severity was recorded by nuclear opalescence (NO) and cortical cataract (CC) degree according to LOCS III. Cumulative dissipated energy (CDE) during phacoemulsification was recorded to verify the severity of the cataract. As a result, we found a moderate correlation between AqTAC and CDE (p < 0.001). In addition, we found AqTAC independently associated with the CDE when analyzed by multivariate linear regression (p < 0.001). AqTAC also negatively correlated to cataract severity when measured by NO and CC (p = 0.012 in NO grade 3 vs. grade 1; p = 0.012 in CC grade 2 vs. grade 1; p < 0.001 in CC grade 3 vs. grade 1). We further found AqAA provided 71.9 ± 13.5% of AqTAC, and showed a high correlation (rho = 0.79, p < 0.001). In conclusion, we found a significant correlation between AqTAC/AqAA and cataract severity measured by CDE. The correlation was superior to the correlation between LOCS III and CDE. Aqueous humor TAC owns the potential to assess cataracts in an objective and quantitative way.

10.3390/antiox11020397

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Citations (12)