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Date information publishing in grid-GIS, one of the key technologies of Grid-GIS realization,is publishing the stored date information in GIS database with the means of Grid. Agent and Middleware provide basis for of the Grid-GIS realization.In this Paper,the character of data information Publishing in Grid-GIS was discussed. According to the character of Grid-GIS, a new method,using the Agent and Middleware to realize the Publishing of date information,was a1so discussed.In order to achieve this purpose the authors designed such Agents as Longin Manager agent,Grid-Web Interface agent,Messenger Agent,Task Manager Agent and Database Interface agent.
A novel cold-living, Gram-stain-negative, yellow-pigmented and obligately aerobic bacterium designated strain 0563T was isolated from the China No. 1 glacier. Phylogenetic analysis showed that strain 0563T was a member of the genus Flavobacterium, sharing the highest 16S rRNA gene sequence similarities with Flavobacterium limicola JCM 11473T (98.5 %) and Flavobacterium psychrolimnae NBRC 102679T (98.2 %). The genomic DNA G+C content was 34.8 mol%. Strain 0563T displayed the common phenotypic and chemotaxonomic features of the genus Flavobacterium, containing menaquinone-6 (MK-6) as the major quinone and iso-C15 : 0, iso-C15 : 0 3-OH, summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c), C15 : 1 ω6c, iso-C15 : 1 G and iso-C16 : 0 3-OH as the major fatty acids. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, the novel species Flavobacterium tiangeerense sp. nov. is proposed; the type strain is 0563T (=CGMCC 1.6847T =JCM 15087T).
Background.Tetrataenium candicans is a traditional Chinese folk herbal medicine used in the treatment of asthma and rheumatic arthritis.Alongside several Tordyliinae species with fleshy roots, it is also regarded as a substitute for a Chinese material medicine called 'Danggui'.However, a lack of sufficient sampling and genomic information has impeded species identification and the protection of wild resources.Methods.The complete chloroplast genomes of T. candicans from two populations, Tetrataenium yunnanense and Semenovia transilliensis, were assembled from two pipelines using data generated from next generation sequencing (NGS).Pseudogenes, inverted repeats (IRs) and hyper-variable regions were located by Geneious 11.1.5.Repeat motifs were searched using MISA and REPuter.DNA polymorphism and segment screening were processed by DNAsp5, and PCR product was sequenced with Sanger's sequencing method.Phylogeny was inferred by MEGA 7.0 and PhyML 3.0.Results.The complete chloroplast genomes of T. candicans from two populations, T. yunnanense and S. transilliensis, were 142,261 bp, 141,985 bp, 142,714 bp and 142,145 bp in length, respectively, indicating conservative genome structures and gene categories.We observed duplications of trnH and psbA caused by exceptional contractions and expansions of the IR regions when comparing the four chloroplast genomes with previously published data.Analyses on DNA polymorphism located 29 candidate cp DNA barcodes for the authentication of 'Danggui' counterfeits.Meanwhile, 34 hypervariable markers were also located by the five Tordyliinae chloroplast genomes, and 11 of them were screened for population genetics of T. candicans based on plastome information from two individuals.The screening results indicated that populations of T.candicans may have expanded.Phylogeny inference on Apiaceae species by CDS sequences showed most lineages were well clustered, but the five Tordyliinae species failed to recover as a monophyletic group, and the phylogenetic relationship between tribe Coriandreae, tribe Selineae, subtribe Tordyliinae and Sinodielsia clade remains unclear.Discussion.The four chloroplast genomes offer valuable information for further research on species identification, cp genome structure, population demography and phylogeny in Apiaceae subfamily Apioideae.
Codonopsis atriplicifolia (Campanulaceae) from western Sichuan, China, is described here as a new species, which is similar to C. subscaposa in a number of characters. The results of molecular phylogenetic analysis of 25 species of Codonopsis based on chloroplast DNA fragments (matK, petD with petB-petD, rbcL) show that C. atriplicifolia is closely related to C. farreri. In morphology, the new species is distinctly different from the allied species by its linear-lanceolate leaves with subentire to pinnatipartite margins.
Human P-glycoprotein encoded by the ATP-binding cassette sub-family B member 1 (ABCB1) gene is expressed in the blood-brain barrier. ABCB1 protects the brain from many drugs and toxins such as glucocorticoids through the efflux pump. Recent evidence suggests that a specific allele of the ABCB1 gene confers susceptibility to major depressive disorder (MDD) in the Japanese population. The aim of this study was to explore the association of ABCB1 gene polymorphisms with MDD in a local Chinese Han population.Two hundred and ninety-two MDD patients and 208 unrelated individuals were matched by age and sex and examined using a case-control design. Six single nucleotide polymorphisms (SNPs) of the ABCB1 gene, including rs1045642, rs2032583, rs2032582, rs2235040, rs1128503, and rs2235015, were genotyped by ligase detection reaction and multiplex polymerase chain reaction. Linkage disequilibrium and haplotype analysis were investigated in the two study groups.Significant protection for MDD individuals carrying the TG haplotype of rs1045642-rs2032582 was observed (odds ratio 0.470, 95% confidence interval 0.251-0.897, P=0.01). The rs2032582 (G2677T) and rs1128503 (C1236T) SNPs of ABCB1 showed nominal associations with MDD; the other four SNPs of the ABCB1 gene were not associated with MDD.Chinese individuals carrying the TG haplotype of rs1045642-rs2032582 had a nearly 53% lower risk of developing MDD. To the best of our knowledge, this is the first report to analyze the effect of ABCB1 polymorphism on the risk of MDD in a Chinese population.
Angelica nitida is an endemic species in China. The complete chloroplast genome sequence of A. nitida was generated by de novo assembly using whole genome next generation sequencing. The complete chloroplast genome was 146,512bp in length and constructed out of four parts – a large single copy (LSC) region of 93,298bp, a small single copy (SSC) region of 18,068bp and two inverted repeat (IRa and IRb) regions of 17,573bp each. The genome annotation predicted a total of 113 genes, including 80 protein-coding genes, 29 tRNA genes, and 4 rRNA genes. Phylogenetic analysis with the reported chloroplast genomes revealed that A. nitida is most closely related to Angelica dahurica.
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