OBJECTIVE To study the immunomodulatory effect of Part III of Cynomorium songaricum Rupr. on the immunosuppressive mice. METHODS The immunity-deficiency model was induced by intraperitoneal injection of cyclophosphamide (CTX) at the dose of 100 mg/kg in mice; all the animals were divided into normal control group, immunity-deficiency model group, Part III treated group (300 mg/kg) and positive control group (TSPG, 300 mg/kg). The hemogram of peripheral blood, the index of immune organs, the phagocytosis activity of macrophage, the content of serum hemolysin were measured. RESULTS The index of organs, the phagocytosis activity of macrophage and the content of serum hemolysin in the model group increased after administrated of Part III. CONCLUSION Part III from Cynomorium songaricum Rupr. has protective effect on the immunosuppressive mice, which may be related to the increasing of humoral immunity and nonspecific immunity.
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Abstract Background: Leymus chinensis is one of most valuable forage on the native grassland for its higher nutrition content. But the local farmer often gained the worse Leymus chinensis hay due to the unfavourable harvest stage, the nutrition of Leymus chinensis was decreasing from jointing stage to solid stage for its growth characteristics , and the results showed that the crude protein (CP) decreased and the fiber content increased which will caused the nutrition of forage reduced. However, the reason for the nutrient changes in Leymus chinensis has not yet explained at the metabolic level. Results: In this research, the Leymus chinensis was harvested at two growth stage( jointing stage and solid stage). Metabolomics was used to analyze the expression changes of metabolites in the whole plant of Leymus chinensis at different harvest stage, and the metabolites were analyzed based on LC-MS/MS untargeted metabolomics. A total of 55 metabolic pathways and 119 metabolites were related to the nutritional change in Leymus chinensis . Most of the metabolites are involved in metabolic processes, including amino acid metabolism, carbohydrate metabolism and acid metabolism biosynthesis. Under the action of EBF1/2 and EIN3, cysteine participates in the ubiquitin-mediated protein degradation pathway. In solid stage, the increasing content of cysteine directly promotes the ubiquitin-mediated protein degradation rate, which is manifested reduce the synthesis of crude protein (CP) in Leymus chinensis , resulting in the decreasing of Leymus chinensis nutrient quality. Carbohydrate metabolism provides raw materials for the synthesis of hemicellulose, leading to the increasing of hemicellulose. In addition, phenylalanine provides the necessary conditions for the synthesis of lignin. These are the internal factors that lead to the decline of quality of Leymus chinensis. Conclusions: This study analyzed the elucidate the relationship between the reduction in the nutritional value of Leymus chinensis and complex biological processes by the metabolomic,This study will provides a theoretical basis for producing high-quality Leymus chinensi s hay and sets the stage for further research.
A comparative study of cold crystallization behavior in poly(L-lactide) (PLLA) annealed below and just above the glass transition temperature (T(g)) has been conducted. Annealing benefits the generation of local order and the subsequent cold crystallization process, which becomes significant in PLLA annealed just above T(g). Surprisingly, morphological observation reveals high density nuclei in PLLA annealed below T(g), contrary to its relatively slow crystallization kinetics. This unusual crystallization behavior in physically aged PLLA arises from the retarded crystal growth rate because of incomplete recovery of reduced segmental mobility above T(g). In contrast, annealing just above T(g) has little influence on the crystal growth rate, and the increased nucleation density alone accounts for the accelerated crystallization rate.
Bacterial laccases are ideal alternatives of fungal laccases for specific industrial applications due to specific characteristics such as alkalescence dependence and high chloride tolerance. However, some bacterial laccases presented as inclusion bodies when expressing in Escherichia coli and showed thermal instability. In this study, rational design was employed to enhance the solubility and the thermostablity of the bacterial laccase Lac15-His6 based on the crystal structure obtained previously. After deletion of His-tag and residues323–332, the obtained Lac15D was completely expressed in soluble form even at a higher temperature of 28°C, compared to only 50% of Lac15-His6 expressed solubly at 16°C. It showed a two-time higher activity at temperatures lower than 35°C and a half-life increasing from 72 min to 150 min at 45°C. When used in chromogenic reactions, Lac15D showed constant activity toward dye precursors and their combinations under alkaline conditions, demonstrating its application potential in hair coloring biotechnology.
Abstract Introduction Laccase is a copper-containing polyphenolic oxidase widely found in bacteria, archaea, fungi, animals, and plants. As a green biocatalyst with considerable potential for numerous environmental and industrial applications, the enzyme production efficiency of laccase in nature is low, and the cost is high. Purpose To examine the characterization and potential applications of laccase in this study, a novel laccase from Halomonas alkaliantarctica (LacHa) was cloned and heterologously expressed in Escherichia coli . Results To achieve heterologous and efficient laccase expression, a bacterial laccase gene designed as LacHa from Halomonas alkaliantarctica of deep sea was cloned and expressed in E. coli . The results showed that the optimum temperature and pH of the enzyme reaction were 45°C and 7.5. The 100 μM Cu 2+ and Fe 2+ ions had the strongest stimulatory effect on laccase activity, the surface-active agent SDS and organic solvent 5% ethanol had opposite effect. EDTA, and 5% DMSO have no effect on LacHa activity. The activity of LacHa was enhanced 1.5 fold by chloride at concentrations lower than 500 mM, and 57.6% of its initial activity remained in the reaction system containing 1000 mM NaCl. Furthermore, LacHa showed decolorization rates ranging from 90.28% to 100% for indigo carmine and two azo dyes without mediators, with wide pH (5.0-9.0) and temperature (25-65°C) ranges. Conclusions In the present study, LacHa was expressed and showed unusual properties, suggesting its great application potential in the treatment of textile industries or environmental applications.
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Abstract Introduction Laccase is a copper-containing polyphenolic oxidase widely found in bacteria, archaea, fungi, animals, and plants. As a green biocatalyst with considerable potential for numerous environmental and industrial applications, the enzyme production efficiency of laccase in nature is low, and the cost is high. Purpose To examine the characterization and potential applications of laccase in this study, a novel laccase from Halomonas alkaliantartica (LacHa) was cloned and heterologously expressed it in Escherichia coli . Results To achieve heterologous and efficient laccase expression, a bacterial laccase gene designed as LacHa from Halomonas alkaliantartica of deep sea was cloned and expressed in E. coli . The results showed that the optimum temperature and pH of the enzyme reaction were 45 °C and 7.5. The 100 μM Cu 2+ and Fe 2+ ions had the strongest stimulatory effect on laccase activity, the surface-active agent SDS and organic solvent 5% ethanol had opposite effect. EDTA, and 5% DMSO have no effect on LacHa activity. The activity of LacHa was enhanced 1.5-fold by chloride at concentrations lower than 500 mM, and 57.6% of its initial activity remained in the reaction system containing 1000 mM. NaCl. Furthermore, LacHa showed decolorization rates ranging from 90.28 to 100% for indigo carmine and two azo dyes without mediators, with wide pH (5.0–9.0) and temperature (25–65 °C) ranges. Conclusions In this study, LacHa was expressed and showed unusual properties, indicating its great application potential in textile industries or environmental fields.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)