I.W. Lesslie

Weybridge Community Hospital

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Co-Authors

C. Nancy Hebert

Central Veterinary Research Laboratory

Catherine A. Herbert

University of Sydney

K. J. Birn

Central Veterinary Research Laboratory

Christine L. Miller

University of Wyoming

Ian Sutherland

Sogang University

C.J. Stewart

Oklahoma State University Center for Health Sciences

Patrick M. Stuart

Washington University in St. Louis

P. D’Arcy Hart

The Francis Crick Institute

J Augier

Institut Pasteur

R Dobbelaer

Institut Scientifique de Santé Publique

Cooperative Institutions

Central Veterinary Research Laboratory

Ministry of Agriculture, Fisheries and Food

Veterinary Medicines Directorate

Ministère de l'Agriculture et de la Souveraineté alimentaire

University College London

Medical Research Council

University of New England

Queen Elizabeth Hospital Birmingham

St Helens Hospital

Statens Serum Institut

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Mycobacterium avium infections in cattle and pigs in Great Britain

Tubercle (1970)

I.W. Lesslie K. J. Birn

Mycobacterium avium complex

10.1016/0041-3879(70)90011-5

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Citations (22)

Purified protein derivatives prepared from non-tuberculous mycobacteria including Mycobacterium johnei.

I.W. Lesslie

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Métodos para el diagnóstico bacteriológico de la tuberculosis bovina

Isabel N Kantor I.W. Lesslie

La inoculacion al cobayo se ha considerado tradicionalmente como el metodo de eleccion para el diagnostico bacteriologico de la infeccion por Mycobacterium bovis. No obstante, en los ultimos anos, en los laboratorios de bacteriologia de la tuberculosis se ha generalizado el empleo de los medios de cultivo a base de piruvato (1, 2), que permiten un desarrollo eugonico del bacilo bovino. Por otra parte, la inoculacion al cobayo en la practica de rutina de un laboratorio, presenta ciertas exigencias de orden economico tales como la adquisicion de animales, instalaciones y equipo, personal y mantenimiento, que resultan muy elevadas al compararlas con las que se requieren para la realizacion de cultivos. Al considerar estos hechos, los autores compararon los resultados obtenidos con uno y otro procedimiento, mediante el empleo de materiales de origenes distintos.

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The Use of Dilute Tuberculins for Testing Cattle

British Veterinary Journal (1965)

I.W. Lesslie C. Nancy Hebert

Tuberculin test

Skin test

10.1016/s0007-1935(17)41010-4

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Citations (7)

The specificity and stability of old tuberculin and tuberculin PPD

Tubercle (1968)

I.W. Lesslie

10.1016/0041-3879(68)90050-0

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Citations (5)

Practical application of bovine tuberculin PPD in testing cattle in Great Britain

Veterinary Record (1976)

I.W. Lesslie C. Nancy Hebert G. Frerichs

A national tuberculin testing trial was carried out in 8151 cattle in 132 herds due for normal herd testing and distributed throughout the 11 animal health administrative regions of England, Scotland and Wales. The numbers selected from each region were in approximately equal proportions to the total cattle populations of the regions and comprised a representative sample of the national herd. The standard intradermal comparative tuberculin test using Weybridge avian and human purified protein derivatives (PPD) was carried out on one side of the neck, and Rotterdam bovine PPD (1 X 0 mg per ml) was injected on the opposite side in the usual 'mammalian' site. Of the cattle tested, 9 X 4 per cent were classified as positive reactors to a single intradermal injection of the bovine tuberculin applying the official EEC interpretation for tuberculin tests. A single intradermal injection of human PPD would have resulted in a significantly higher proportion (10 X 4 per cent) of cattle being designated for removal. The rules of interpretation developed by Lesslie and Hebert (1975) for a comparative tuberculin test using avian and bovine PPDs, when applied to this sample, classified 0 X 80 per cent as inconclusive reactors compared with 0 X 58 per cent for the avian/human comparative test. The avian/bovine test also classified as positive reactors 0 X 12 per cent of the cattle tested, whilst the avian/human test classified as positive 0 X 05 per cent of cattle in routine herd tests during the three-year period 1972 to 1974.

Tuberculin test

Bovine tuberculosis

Animal health

Milking

10.1136/vr.98.9.170

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Citations (5)

The International Reference Preparations of Clostridium welchii (C. perfringens) beta and epsilon toxoids.

PubMed (1978)

Iain Davidson Amanda Gray C. Nancy Hebert I.W. Lesslie

The Central Veterinary Laboratory, Weybridge, England was requested by the WHO Expert Committee on Biological Standardization to obtain suitable materials for international standards for Clostridium welchii (C. perfringens) beta and epsilon toxoids and to arrange collaborative assays. Preparations were obtained and dispensed as freeze-dried toxoids in ampoules. The toxoids were assayed by nine laboratories in eight countries. On the basis of the results obtained, the materials have been established as the International Reference Preparations of Clostridium welchii (C. perfringens) Beta and Epsilon Toxoids.

Clostridium perfringens

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The relationship between length of incubation, bacterial growth and tuberculin yield of a strain of mycobacterium tuberculosis

Tubercle (1975)

I.W. Lesslie D. A. Denham C. Nancy Hebert

Incubation period

Bacterial growth

Microbiological culture

Tuberculin test

10.1016/0041-3879(75)90006-9

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Citations (3)

Comparison of the specificity of human and bovine tuberculin PPF for testing cattle. 3. National trial in Great Britain

Veterinary Record (1975)

I.W. Lesslie Catherine A. Herbert

A field trial on a country-wide basis was undertaken to compare the specificity for bovine tuberculosis of single and comparative tuberculin tests in cattle using either Weybridge human or Weybridge bovine PPD. The tests were made on 10,305 cattle in 179 herds distributed throughout all regions of England, Scotland and Wales. Results showed that a comparative tuberculin test using avian PPD with either human or bovine PPD had a much higher efficiency than a single injection of mammalian tuberculin in the neck of cattle, and confirmed that a comparative test is still essential in the British environment. Weybridge bovine PPD gave significantly better discrimination between tuberculous and non-tuberculous cattle than Weybridge human PPD when used together with avian PPD in a comparative tuberculin test. The diameter of induration gave an absolute measure of the extent of oedema, if present, and induration diameter used in conjunction with skin thickening increased the sensitivity and specificity of the test. Rules of interpretation were developed and are presented for an intradermal comparative tuberculin test in cattle using Weybridge avian and bovine PPDs.

Bovine tuberculosis

Tuberculin test

10.1136/vr.96.15.338

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Citations (32)

Comparison of the specificty of human and bovine tuberculin PPD for testing cattle. 1--Republic of Ireland

Veterinary Record (1975)

I.W. Lesslie Catherine A. Herbert K. Burn B. MacClancy W. Donnelly

A tuberculin testing trial in cattle was carried out in the Republic of Ireland to compare the specificity for bovine tuberculosis of a human purified protein derivative (PPD) tuberculin (Weybridge) with that of a bovine PPD (Rotterdam), and to determine whether discrimination between specific and non-specific reactions to mammalian tuberculin is better with doses of tuberculins smaller than those traditonally used for testing cattle. Tests were carried out in 510 cattle, 395 of which were shown by post mortem examination to be tuberculous and 115 non-tuberculous. Three dilutions at five-fold intervals of both mammalian tuberculins were used together with two dose levels of avian tuberculin PPD (Weybridge), and all reactions were measured both by increase in skin fold thickness and by diameter of induration. In the environment of this trial, the bovine PPD was shown to be more specific for bovine tuberculosis than the human PPD, and particularly in differentiating from "skin tuberculosis". There was no indication of greater specificity at lower doses of tuberculin. Measurement of induration diameter proved a satisfactory alternative method of reading tuberculin reactions in cattle under field conditions.

Bovine tuberculosis

Serial dilution

Purified protein derivative

10.1136/vr.96.15.332

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Citations (25)