Roberta Galli

TU Dresden

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Ortrud Uckermann

University Hospital Carl Gustav Carus

Edmund Koch

TU Dresden

Gerald Steiner

University Hospital Carl Gustav Carus

Gabriele Schackert

TU Dresden

Matthias Kirsch

TU Dresden

Tareq A. Juratli

University Hospital Carl Gustav Carus

Hermann Ehrlich

Adam Mickiewicz University in Poznań

Kathrin Geiger

Johns Hopkins University

Achim Temme

National Center for Tumor Diseases

Dietmar Krex

TU Dresden

Cooperative Institutions

TU Dresden

157

University Hospital Carl Gustav Carus

108

TU Bergakademie Freiberg

Klinik und Poliklinik für Psychotherapie und Psychosomatik

Universidade Federal do Paraná

The Ohio State University

Sapienza University of Rome

National Center for Tumor Diseases

Hospital de Clínicas Universidade Federal do Paraná

German Cancer Research Center

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Isolation and identification of chitin in three-dimensional skeleton of Aplysina fistularis marine sponge

International Journal of Biological Macromolecules (2013)

Marcin Wysokowski Vasilii V. Bazhenov Mikhail V. Tsurkan Roberta Galli Allison L. Stelling

Sponge

Chitinase

10.1016/j.ijbiomac.2013.08.039

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Citations (97)

Synthesis of nanostructured chitin–hematite composites under extreme biomimetic conditions

RSC Advances (2014)

Marcin Wysokowski Mykhailo Motylenko Juliane Walter Grzegorz Lota Jarosław Wojciechowski

Chitin of poriferan origin is a unique and thermostable biological material. It also represents an example of a renewable materials source due to the high regeneration ability of Aplysina sponges under marine ranching conditions. Chitinous scaffolds isolated from the skeleton of the marine sponge Aplysina aerophoba were used as a template for the in vitro formation of Fe2O3 under conditions (pH ∼ 1.5, 90 °C) which are extreme for biological materials. Novel chitin–Fe2O3 three dimensional composites, which have been prepared for the first time using hydrothermal synthesis, were thoroughly characterized using numerous analytical methods including Raman spectroscopy, XPS, XRD, electron diffraction and HR-TEM. We demonstrate the growth of uniform Fe2O3 nanocrystals into the nanostructured chitin substrate and propose a possible mechanism of chitin–hematite interactions. Moreover, we show that composites made of sponge chitin–Fe2O3 hybrid materials with active carbon can be successfully used as electrode materials for electrochemical capacitors.

Sponge

Hydrothermal Synthesis

10.1039/c4ra10017d

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Citations (55)

Riduzione di ocratossina A nei vini mediante batteri lattici

Angela Silva Maria Daria Fumi Roberta Galli

Source

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Citations (1)

Characterization of signaling pathways leading to Fas expression induced by TNF‐α: pivotal role of NF‐κB

The FASEB Journal (2004)

Donatella Starace Anna Riccioli Alessio D’Alessio Claudia Giampietri Simonetta Petrungaro

TNF-alpha is known to induce a strong up-regulation of Fas expression in mouse Sertoli cell cultures, leading to their apoptosis triggered by effector FasL-bearing cells. These data suggest that increased Fas expression on the cell surface might be a key event in the pathogenesis of autoimmune orchitis, by inducing a leakage of the blood-tubular barrier as a consequence of Sertoli cell apoptosis. In the present paper, we have investigated the signal transduction mechanisms involved in the regulation of Fas expression induced by TNF-alpha in mouse Sertoli cells. We studied the role of the transcription factor NF-kappaB and of MAPKs in regulating Fas expression. By using Sertoli cells transfected with a NF-kappaB Luc reporter gene, we proved that TNF-alpha activates the IkappaB/NF-kappaB system. Moreover, the use of the proteasome inhibitor lactacystin led us to demonstrate that NF-kappaB is required for TNF-alpha mediated Fas expression. By using specific inhibitors for each MAPK, we confirmed the pivotal role of the IkappaB/NF-kappaB system by demonstrating that ERKs, p38, and JNK are not involved in Fas up-regulation by TNF-alpha. The comprehension of these pathways could be relevant to the knowledge of the pathogenesis of autoimmune disorders in immune privileged districts of the body.

Fas ligand

Lactacystin

10.1096/fj.04-2726fje

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Citations (31)

Creation of a 3D Goethite–Spongin Composite Using an Extreme Biomimetics Approach

Biomimetics (2023)

Anita Kubiak Alona Voronkina Martyna Pajewska-Szmyt Martyna Kotula Bartosz Leśniewski

The structural biopolymer spongin in the form of a 3D scaffold resembles in shape and size numerous species of industrially useful marine keratosan demosponges. Due to the large-scale aquaculture of these sponges worldwide, it represents a unique renewable source of biological material, which has already been successfully applied in biomedicine and bioinspired materials science. In the present study, spongin from the demosponge Hippospongia communis was used as a microporous template for the development of a new 3D composite containing goethite [α-FeO(OH)]. For this purpose, an extreme biomimetic technique using iron powder, crystalline iodine, and fibrous spongin was applied under laboratory conditions for the first time. The product was characterized using SEM and digital light microscopy, infrared and Raman spectroscopy, XRD, thermogravimetry (TG/DTG), and confocal micro X-ray fluorescence spectroscopy (CMXRF). A potential application of the obtained goethite-spongin composite in the electrochemical sensing of dopamine (DA) in human urine samples was investigated, with satisfactory recoveries (96% to 116%) being obtained.

Biopolymer

Biomimetic Synthesis

Thermogravimetry

Biomimetics

10.3390/biomimetics8070533

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Citations (3)

Micro-granulate cork stoppers: study on overall and specific migration

Milena Lambri Roberta Galli Michele Monti Fabrizio Torchio Dante Marco De Faveri

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Citations (0)

Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris

Journal of Structural Biology (2013)

Hermann Ehrlich Oksana V. Kaluzhnaya Eike Brunner Mikhail V. Tsurkan Alexander Ereskovsky

Sponge

Biopolymer

10.1016/j.jsb.2013.06.015

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Citations (98)

Current approaches to avoid the culling of day-old male chicks in the layer industry, with special reference to spectroscopic methods

Poultry Science (2017)

M.‐E. Krautwald‐Junghanns K. Cramér Björn Fischer A. Förster Roberta Galli

The negative correlation between fattening and laying performance prevents breeding improvement in both laying performance and meat yield. Therefore, specialized chicken lines have been bred in order to achieve either an efficient production of high-quality eggs or high growth rates. As a result, day-old male chicks are culled in the layer hatchery, which poses animal welfare and ethical problems. Breeding companies, scientific groups, and hatcheries are attempting to resolve this issue, with a common aim to find feasible alternatives for the routine killing of male layer chicks. Some approaches aim to influence the sex ratio, while others target at the economically feasible use of the male layer offspring, such as the fattening of "laying hen brothers" or crossbreedings of layers and broilers to create "dual-purpose chickens." Another approach is the sex determination prior to hatch. One of the prerequisites of in ovo sex determination is a practicable method that can be used in industry. The analysis needs to be rapid, cost-efficient, and highly precise; in addition, negative impacts on hatching rate, animal health, and/or performance parameters should be limited. Furthermore, sex determination should be performed before the sensory nervous system's response of the chick embryo to certain or potentially harmful stimuli is developed, which according to current knowledge is before the d 7 of incubation.

Culling

In ovo

Hatchery

Feather pecking

10.3382/ps/pex389

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Citations (125)

Vernici per ambienti con presenza di alimenti Seconda parte (Coating for food-compatible II Parte)

Claudio Pagella Maria Daria Fumi Roberta Galli

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Raman and autofluorescence spectroscopy for in situ identification of neoplastic tissue during surgical treatment of brain tumors

Research Square (Research Square) (2024)

Ortrud Uckermann Jonathan Ziegler Matthias Meinhardt Sven Richter Gabriele Schackert

Abstract Purpose Raman spectroscopy (RS) is a promising method for brain tumor detection. Near-infrared autofluorescence (AF) acquired during RS provides additional information useful for tumor identification and was investigated in comparison with RS for delineating brain tumors in situ. Methods Raman spectra together with AF were acquired in situ within the solid tumor and at the tumor border during routine brain tumor surgeries (218 spectra; glioma WHO II-III n = 6, GBM n = 10, metastases n = 10, meningioma n = 3). Tissue classification for tumor identification in situ was trained on data acquired ex vivo (375 spectra; glioma/GBM patients n = 20, metastases n = 11, meningioma n = 13, epileptic hippocampi n = 4). Results Both in situ and ex vivo data showed that AF intensity in brain tumors is lower compared to border regions and normal brain tissue. Moreover, a positive correlation was observed between the AF intensity and the intensity of the Raman band of lipids at 1437 cm^{− 1}, while a negative correlation was found with the intensity of the protein band at 1260 cm^{− 1}. The classification of in situ AF and RS datasets matched the surgeon’s evaluation of tissue type with a correct rate of 0.83 and 0.84, respectively. Similar correct rates were achieved in comparison to histopathology of tissue biopsies resected in selected measurement positons (AF: 0.80, RS: 0.83). Conclusions Spectroscopy was successfully integrated into existing neurosurgical workflows and in situ spectroscopic data could be classified based on ex vivo data. RS confirmed its ability in detecting brain tumors, while AF emerged as a competitive method for intraoperative tumor delineation.

Autofluorescence

Ex vivo

Brain tumor

Intensity

Histopathology

Brain tissue

10.21203/rs.3.rs-4730107/v1

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Citations (0)