Neonatal hypoxic-ischemic (HI) encephalopathy (HIE) remains a major cause of mortality and persistent neurological disabilities in affected individuals. At present, hypothermia is considered to be the only applicable treatment option, although growing evidence suggests that cell-based therapy might achieve better outcomes. Dedifferentiated fat (DFAT) cells are derived from mature adipocytes via a dedifferentiation strategy called ceiling culture. Their abundance and ready availability might make them an ideal therapeutic tool for the treatment of HIE. In the present study, we aimed to determine whether the outcome of HIE can be improved by DFAT cell treatment. HI injury was achieved by ligating the left common carotid artery in 7-day-old rat pups, followed by 1-h exposure to 8% O2. Subsequently, the severity of damage was assessed by diffusion-weighted magnetic resonance imaging to assign animals to equivalent groups. 24 h after hypoxia, DFAT cells were injected at 105 cells/pup into the right external jugular vein. To evaluate brain damage in the acute phase, a group of animals was sacrificed 48 h after the insult, and paraffin sections of the brain were stained to assess several acute injury markers. In the chronic phase, the behavioral outcome was measured by performing a series of behavioral tests. From the 24th day of age, the sensorimotor function was examined by evaluating the initial forepaw placement on a cylinder wall and the latency to falling from a rotarod treadmill. The cognitive function was tested with the novel object recognition (NOR) test. In vitro conditioned medium (CM) prepared from cultured DFAT cells was added at various concentrations to neuronal cell cultures, which were then exposed to oxygen-glucose deprivation (OGD). The number of cells that stained positive for the apoptosis marker active caspase-3 decreased by 73 and 52% in the hippocampus and temporal cortex areas of the brain, respectively, in the DFAT-treated pups. Similarly, the numbers of ED-1-positive cells (activated microglia) decreased by 66 and 44%, respectively, in the same areas in the DFAT-treated group. The number of cells positive for the oxidative stress marker 4-hydroxyl-2-nonenal decreased by 68 and 50% in the hippocampus and the parietal cortex areas, respectively, in the DFAT-treated group. The HI insult led to a motor deficit according to the rotarod treadmill and cylinder test, where it significantly affected the vehicle group, whereas no difference was confirmed between the DFAT and sham groups. However, the NOR test indicated no significant differences between any of the groups. DFAT treatment did not reduce the infarct volume, which was confirmed immunohistochemically. According to in vitro experiments, the cell death rates in the DFAT-CM-treated cells were significantly lower than those in the controls when DFAT-CM was added 48 h prior to OGD. The treatment effect of adding DFAT-CM 24 h prior to OGD was also significant. Our results indicate that intravenous injection with DFAT cells is effective for ameliorating HI brain injury, possibly via paracrine effects.
Increasing the energy self-consumption rate is an important challenge for economic improvement in a grid parity environment. Here, the construction of a power supply system that combines solar power generation and a storage battery system is detailed and the increase in the renewable energy self-consumption rate obtained by optimizing the charging and discharging of the storage battery is reported.
In this paper we construct a two country model with a final good and an environmental good such as a pollution abatement equipment to examine the formation of comparative advantage based on the international differences of environmental policies and the effects of the emission tax under free trade. The main results of our paper are as follows. First, the subsidy on the environmental good purchase can be a substitute for the emission tax. That is, the government can impose the lower emission tax by raising the subsidy in order to achieve the first best allocation. Second, a raise in the emission tax decreases the relative price of the environmental good while a raise in the subsidy increases the relative price. Hence, ceteris paribus, the country with a higher emission tax or a lower subsidy on the use of the environmental good has a comparative advantage in this good. Third, the effects of the emission tax in one country on the amount of pollution and welfare depends on the specialization pattern under free trade.
A flying head using the switching characteristics of an optically switched laser diode is shown to have an excellent read/write performance and head-disk interface. This head consists of an aluminum nitride slider and a 1.3-microm wavelength InGaAsP laser diode monolithically integrated with a photodetector (LD-PD). A ridged waveguide is fabricated on top of the LD facet to converge the light beam. The lensless flying head has a minimum power of 18 mW for writing information (at 1 MHz) at a medium velocity of 7 m/s. The disk has a SiN protective layer over a SbTeGe phase-change recording medium. The head signal remains good after more than 5 x 10(4) contact start/stop cycles and 4.4 x 10(8) passes in an experimental room environment.
We evaluate the physical properties of volume holographic recording in photopolymer that allow for high performance nonvolatile digital data storage. We identified efficient volume grating formation for photoinitiated cationic-ring- opening polymerization that utilizes unrestricted monomer diffusive transport contributions in the grating development process during and after exposure.
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
