Shihua Yang

South China Agricultural University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Weizhi Ji

Kunming University of Science and Technology

Qi Zhou

Institute of Zoology

Yuyu Niu

Kunming University of Science and Technology

Yunhua Xie

Kunming University of Science and Technology

Chenhui Ding

Sun Yat-sen University

Xiechao He

Chinese Academy of Sciences

Thomas B. Hildebrandt

Leibniz Institute for Zoo and Wildlife Research

Zulqarnain Baloch

Kunming University of Science and Technology

Shaohui Ji

Nanchang Institute of Technology

Qunshan Huang

South China Agricultural University

Cooperative Institutions

Chinese Academy of Sciences

Sun Yat-sen University

Kunming University of Science and Technology

South China Agricultural University

University of Chinese Academy of Sciences

Massachusetts Institute of Technology

Kunming Institute of Zoology

Sichuan University

Center for Excellence in Molecular Plant Sciences

National Center for Gene Research

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Effects of rhFSH regimen and time interval on ovarian responses to repeated stimulation cycles in rhesus monkeys during a physiologic breeding season

Theriogenology (2008)

Shihua Yang Yan Shen Yuyu Niu Thomas B. Hildebrandt Katarina Jewgenow

Regimen

10.1016/j.theriogenology.2008.03.012

Cite

Citations (14)

Neural progenitors derived from monkey embryonic stem cells in a simple monoculture system

Reproductive BioMedicine Online (2009)

Xinjie Chen Tianqing Li Xuemei Li Yunhua Xie Xiangyu Guo

Neuroepithelial cell

Neurosphere

Embryoid body

Progenitor

10.1016/s1472-6483(10)60179-4

Cite

Citations (5)

Orientation-dependent phase transition and dielectric properties of Ba0.85Ca0.15Ti0.9Zr0.1O3 thin films

Journal of Materials Science Materials in Electronics (2012)

Shihua Yang Jinbao Xu Bo Gao Lei Wang Jing Chen

Deposition

10.1007/s10854-012-0787-5

Cite

Citations (7)

Prevalence and risk factors for human papillomavirus infection among Chinese ethnic women in southern of Yunnan, China

The Brazilian Journal of Infectious Diseases (2017)

Zulqarnain Baloch Nafeesa Yasmeen Yuanyue Li Ke Ma Xiaomei Wu

Dai is a major Chinese ethnic minority group residing in rural areas of the southern part of Yunnan. However, no data exist on the Human Papillomavirus (HPV) prevalence and genotype distribution among Dai women.A total of 793 participants (Dai=324, Han=251, other ethnic=218) were included in this study. PCR was performed to detect the HPV-positive samples, and genotyping was performed with an HPV Geno-Array.The overall HPV prevalence was very low among Dai women compared to the others. The prevalence of high-risk-HPV infections was significantly higher (p=0.001) among other ethnic women (22.0%) than that among Han (13.1%) and Dai women (7.1%). The overall HPV, high-risk-HPV, single and multiple infection prevalence among rural women were 12.9%, 12.1%, 12.3%, and 0.5%, respectively. HPV-16 (5.5%) was shown to be the most prevalent genotype, followed by HPV-52 (2.6%) and HPV-58 (2.4%). Urban women had relatively higher rates of overall HPV (16.0%), high-risk-HPV (14.1%), single genotype (11.9%), and multiple genotype (4.1%) infections. In urban women, HPV-52 (3.6%) was the most prevalent genotype, followed by HPV-39 (2.7%) and HPV-16 (1.2%). In the urban area, HPV prevalence was highest in women aged <29 years, but declined with increasing age. However, in rural women, the highest HPV prevalence was observed among women at older age (>50 years). Education and smoking habit were significantly associated with HPV infection among both rural and urban area women (p<0.001).The prevalence and genotype distribution of HPV varied among ethnic women in urban and rural area of Yunnan Province.

Ethnic chinese

10.1016/j.bjid.2017.01.009

Cite

Citations (40)

Complete chloroplast genome sequence of Nicotiana tabacum TN90 (Solanaceae)

Mitochondrial DNA Part B (2016)

Hui Gao Yun-jiao Zhang Yun-chuan Zhao Chang-gui Qiu Jihui Liu

This study reported the complete nucleotide sequence of the Nicotiana tabacum TN90 chloroplast (cp) genome. The cpDNA was 155 992 bp in length and contained 133 individual genes (79 protein encoding genes, 30 tRNA genes and four rRNA genes). Maximum-likelihood (ML) phylogenetic tree for 17 species with Arabidopsis thaliana, Oryza sativa, and Anomochloa marantoidea as an outgroup resulted in a single tree with − lnL =542 222.71, where the Nicotiana tabacum TN90 plastid was clustered with three previous reported Nicotiana species: N. tomentosiformis, N. undulata and N. tabacum. The TN90 variety of tobacco cp genome sequence reported in this study will accelerate tobacco improvement in the future.

10.1080/23802359.2015.1137808

Cite

Citations (2)

Effect of Donor Age on the Developmental Competence of Bovine Oocytes Retrieved by Ovum Pick Up

Reproduction in Domestic Animals (2009)

Lei Su Shihua Yang Xinrong He Xu Li Juan Ma

Contents To study the effect of donor age on oocyte developmental competence and steroid profiles, the crossbred cow (Murray Grey × Brahman) in Yunnan province of China were selected and divided into three groups according to its age. The three groups were young cows (n = 12; 12 months old), middle‐aged cows (n = 15; parity: ≤3 calvings; age: 7–8 years old) and old cows (n = 10; parity: ≥8 calvings; age: ≥15 years old). Cumulus–oocyte complexes (COCs) were collected by 10 consecutive ovum pick up (OPU) sessions with a 4‐day interval between each session, followed by in vitro maturation, fertilization and embryo development. Results showed that cleavage rates (CR) and blastocyst rates (BR) were higher in the young cows than those in the middle‐aged and old cows (p < 0.05). CR and BR from COCs of the first and the fourth OPU sessions were lower than those from other sessions in the young cows and the middle‐aged cows (p < 0.05), whereas the similar phenomenon was not observed in the old cows. Plasma concentrations of oestradiol were higher, and plasma concentrations of progesterone were lower before and during OPU sessions in the young cows compared with those in the same period in the middle‐aged cows or the old cows (p < 0.01). In conclusion, donor age of oocytes could affect developmental competence of oocytes recovered by OPU through the action of steroid hormonal balance on follicle development.

10.1111/j.1439-0531.2009.01349.x

Cite

Citations (39)

[The efficacy of thyroid hormone replacement therapy in patients with hypothyroidism and OSAHS: a Meta analysis].

PubMed (2017)

P P Wang Lei Ma Yunhui Lv Yan Xiang Dang Shao

Objective:To systematically evaluate the impact of thyroid hormone replacement in patients with OSAHS and hypothyroidism.Method:The related case-control study of thyroid hormone replacement in patients with OSAHS and hypothyroidism had been searched from Cochrane Library, PubMed, CNKI, Chinese Biomedical Literature Database, Wanfang Resource Database.The case-control study which were in line with the condition need to be evaluated the quality and to be extracted in literature. The RevMan 5.3 and STATA softwares were applied to carry out the Meta analysis.Result:Seven literatures of case-control study were included. The Meta analysis showed that: After the thyroid hormone replacement, the patient's AHI index, TSH index, LSaO₂ index were significantly improved (P< 0.05). The AHI index statistical results of the patients were [SMD= 3.15, 95%CI: (4.36-1.94), P< 0.000 01; I²= 96%], TSH index were [SMD= 4.15,95%CI: (1.15-7.15), P= 0.007; I²= 99%], LSaO₂ index were [SMD=-1.08, 95%CI: (-1.46--0.70), P< 0.000 01, I²= 68%].Conclusion:The use of thyroid hormone replacement can effectively reduce the patient's hypothyroidism symptoms and AHI symptoms.

Hormone replacement

Thyroid-stimulating hormone

10.13201/j.issn.1001-1781.2017.17.017

Cite

Citations (1)

Dynamic changes in ovarian follicles measured by ultrasonography during gonadotropin stimulation in rhesus monkeys

Theriogenology (2009)

Shihua Yang Xinrong He Yuyu Niu Xin Wang Bin Lu

10.1016/j.theriogenology.2009.04.012

Cite

Citations (5)

Transgenic rhesus monkeys produced by gene transfer into early-cleavage–stage embryos using a simian immunodeficiency virus-based vector

Proceedings of the National Academy of Sciences (2010)

Yuyu Niu Yang Yu Agnieszka Bernat Shihua Yang Xiechao He

The development of transgenic technologies in monkeys is important for creating valuable animal models of human physiology so that the etiology of diseases can be studied and potential therapies for their amelioration may be developed. However, the efficiency of producing transgenic primate animals is presently very low, and there are few reports of success. We have developed an improved methodology for the production of transgenic rhesus monkeys, making use of a simian immunodeficiency virus (SIV)-based vector that encodes EGFP and a protocol for infection of early-cleavage–stage embryos. We show that infection does not alter embryo development. Moreover, the timing of infection, either before or during embryonic genome activation, has no observable effect on the level and stability of transgene expression. Of 70 embryos injected with concentrated virus at the one- to two-cell stage or the four- to eight-cell stage and showing fluorescence, 30 were transferred to surrogate mothers. One transgenic fetus was obtained from a fraternal triple pregnancy. Four infant monkeys were produced from four singleton pregnancies, of which two expressed EGFP throughout the whole body. These results demonstrate the usefulness of SIV-based lentiviral vectors for the generation of transgenic monkeys and improve the efficiency of transgenic technology in nonhuman primates.

Simian immunodeficiency virus

10.1073/pnas.1006563107

Cite

Citations (91)

MAMU-AG TRANSCRIPTION IN PREIMPLANTATION EMBRYOS DERIVED FROM SCNT AND IVF IN RHESUS MONKEY

Biology of Reproduction (2007)

Yuyu Niu Shihua Yang Jifeng Yang Xiechao He X.S. Tang

Mamu -AG is considered to be a functional homolog of human leukocyte antigen (HLA) -G, a nonclassical class I major histocompatibility complex molecule with a plausible involvement in maternal immune reponse during pregnancy. The objective of this study was to investigate Mamu-AG transcription in rhesus monkey preimplantation embryos derived from in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT). With a single preimplantation embryo RT-PCR assay, Mamu-AG mRNA was examined in the two kinds of 8-cell stage (collected on Day2, n=5/each kind of embryo, or Day3, n=3), morula (Day5, n=3) and blastocyst (Day6, n=7 or Day7, n=5). Similarly, in the both kinds of embryos, Mamu-AG mRNA can not be detected in all of the tested 8-cell stage and morula. In IVF embryos, transcripts were detected in the blastocysts collected either on Day6 (7/7, 100%) or on Day7 (5/5, 100%). In SCNT embryos, however, transcripts were only detected in the blastocysts collected on Day7 (5/5, 100%), but not on Day6 (0/7). These results indicated that Mamu-AG is expressed at the mRNA levels by all of rhesus IVF blastocyst and some of SCNT blastocyst. The presence of Mamu-AG mRNA may be delayed in SCNT embryos. This could result from the abnormal nuclear reprogramming process in SCNT embryos and may be contribute to failure in its implantation. (poster)

Inner cell mass

Reprogramming

10.1093/biolreprod/77.s1.164b

Cite

Citations (0)