Renqiang Yuan

The Synergetic Innovation Center for Advanced Materials

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Liqin Ge

State Key Laboratory of Digital Medical Engineering

Delin Mo

Jiangsu Cancer Hospital

Ning Yang

State Key Laboratory of Digital Medical Engineering

Yaosheng Chen

Sun Yat-sen University

Shufang Cai

Wenzhou University

Qianli Zhang

China Pharmaceutical University

Zonghao Liu

State Key Laboratory of Digital Medical Engineering

Yaping Nie

Hubei University of Technology

Xumeng Zhang

Zhongkai University of Agriculture and Engineering

Luxi Chen

Guangdong Pharmaceutical University

Cooperative Institutions

Sun Yat-sen University

Southeast University

Sichuan Agricultural University

State Key Laboratory of Digital Medical Engineering

Ministry of Agriculture and Rural Affairs

Chinese Academy of Sciences

Sichuan University

Chinese Academy of Medical Sciences & Peking Union Medical College

Fudan University

Zhongda Hospital Southeast University

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Integrated miRNA–mRNA transcriptomic analysis reveals epigenetic-mediated embryonic muscle growth differences between Wuzhishan and Landrace pigs1

Journal of Animal Science (2019)

Xumeng Zhang Shufang Cai Luxi Chen Renqiang Yuan Yaping Nie

Pig is one of the major dietary protein sources for human consumption, from which muscle is the largest protein origin. However, molecular mechanisms concerning early porcine embryonic muscle development distinctions between pig breeds are still unclear. In this study, an integrated analysis of transcriptome and miRNAome was conducted using longissimus dorsi muscle of 4 early embryonic stages around the primary myofiber formation time (18-, 21-, 28-, and 35-d post coitus) from 2 pig breeds (Landrace [LR] and Wuzhishan [WZS]) differing in meat mass. The global miRNA/mRNA expression profile showed that WZS prepared for myogenic developmental processes earlier than LR. After identifying and analyzing the interaction network of top 100 up-/down-regulated miRNA and their target genes, we were able to find 3 gene clusters: chromatin modification-related (Chd2, H3f3a, Chd6, and Mll1), myogenesis-related (Pax3, Pbx1, Mef2a, and Znf423), and myosin component-related (Mylk, Myo5a, Mylk4, Myh9, and Mylk2) gene clusters. These genes may involve in miRNA-gene myogenic regulatory network that plays vital role in regulating distinct early porcine embryonic myogenic processes between LR and WZS. In summary, our study reveals an epigenetic-mediated myogenic regulatory axial that will help us to decipher molecular mechanisms concerning early porcine embryonic muscle development distinctions between pig breeds.

PAX3

10.1093/jas/skz091

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Citations (17)

Gallol-based constant underwater coating adhesives for severe aqueous conditions

Colloids and Surfaces A Physicochemical and Engineering Aspects (2021)

Ning Yang Renqiang Yuan Dan You Qianli Zhang Jieran Wang

Leakage (economics)

10.1016/j.colsurfa.2021.127948

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Citations (9)

Adhesion-enhanced coral cells with self-healing coating

Colloids and Surfaces A Physicochemical and Engineering Aspects (2021)

Liqin Ge Chenxi Luo Dan You Ning Yang Qianli Zhang

10.1016/j.colsurfa.2021.127337

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Citations (3)

The SNPs in myoD gene from normal muscle developing individuals have no effect on muscle mass

BMC Genomic Data (2019)

Suying Ding Yaping Nie Xumeng Zhang Xiaohong Liu Chen Wang

Myogenic Differentiation 1 (MyoD) is a crucial master switch in regulating muscle-specific gene transcription. Forced expression of myoD is equipped to induce several cell lineages into myoblast, which then differentiate and fuse into myotube. Pig is one of the most significant livestock supplying meat, and has been classified into lean, fat and miniature pig breeds. However, the mechanisms underlying muscle mass variation among different pig breeds have remained unclear. Considering the important effect of MyoD on muscle development, it remains to be investigated whether the difference in muscle mass is caused by its single nucleotide polymorphisms (SNPs) which are the major differences among pig breeds at DNA level. In this study, we identified the locations of porcine myoD regulatory regions including proximal regulatory region (PRR), distal regulatory region (DRR), and core enhancer (CE) region. There are 8 SNPs in the regulatory regions and 6 SNPs in gene body region, which were identified from lean, fat and miniature pig populations. However, these SNPs have no effects on its temporal expression and transcriptional activity which might lead to the distinction in postnatal muscle mass. In addition, overexpression of myoD clones across from amphibious to mammals including xenopus tropicalis, chicken, mouse and pig whose gene identities vary from 68 to 84%, could promote myogenesis in NIH3T3 fibroblasts cells. These results proved that myoD nucleotide variations from different pig populations have no effect on muscle mass, suggesting that the function of myoD is highly conserved not only among different pig breeds, but also across different species. Thus, it would be futile to discover SNPs affecting muscle mass in pig populations with normal muscle development.

PITX2

10.1186/s12863-019-0772-6

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MLL1 promotes myogenesis by epigenetically regulating Myf5

Cell Proliferation (2019)

Shufang Cai Qi Zhu Cilin Guo Renqiang Yuan Xumeng Zhang

Abstract Objectives Mixed lineage leukaemia protein‐1 (MLL1) mediates histone 3 lysine 4 (H3K4) trimethylation (me3) and plays vital roles during early embryonic development and hematopoiesis. In our previous study, we found its expression was positively correlated with embryonic myogenic ability in pigs, indicating its potential roles in mammalian muscle development. The present work aimed to explore the roles and regulation mechanisms of MLL1 in myogenesis. Materials and methods The expression of MLL1 in C2C12 cells was experimentally manipulated using small interfering RNAs (siRNA). 5‐ethynyl‐2′‐deoxyuridine (EdU) assay, cell cycle assay, immunofluorescence, qRT‐PCR and Western blot were performed to assess myoblast proliferation and differentiation. Chromatin immunoprecipitation assay was conducted to detect H3K4me3 enrichment on myogenic factor 5 ( Myf5 ) promoter. A cardiotoxin (CTX)‐mediated muscle regeneration model was used to investigate the effects of MLL1 on myogenesis in vivo. Results MLL1 was highly expressed in proliferating C2C12 cells, and expression decreased after differentiation. Knocking down MLL1 suppressed myoblast proliferation and impaired myoblast differentiation. Furthermore, knockdown of MLL1 resulted in the arrest of cell cycle in G1 phase, with decreased expressions of Myf5 and Cyclin D1. Mechanically, MLL1 transcriptionally regulated Myf5 by mediating H3K4me3 on its promoter. In vivo data implied that MLL1 was required for Pax7‐positive satellite cell proliferation and muscle repair. Conclusion MLL1 facilitates proliferation of myoblasts and Pax7‐positive satellite cells by epigenetically regulating Myf5 via mediating H3K4me3 on its promoter.

MYF5

H3K4me3

C2C12

Myogenic regulatory factors

Chromatin immunoprecipitation

10.1111/cpr.12744

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Citations (21)

Fabrication of l-menthol contained edible self-healing coating based on guest-host interaction

Colloids and Surfaces A Physicochemical and Engineering Aspects (2020)

Yifan Yang Jiaoyu Ren Chenxi Luo Renqiang Yuan Liqin Ge

Sodium alginate

10.1016/j.colsurfa.2020.124743

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Citations (25)

Additional file 1: of The SNPs in myoD gene from normal muscle developing individuals have no effect on muscle mass

Figshare (2019)

Suying Ding Yaping Nie Xumeng Zhang Xiaohong Liu Chen Wang

Table S1. The information of all samples. (XLSX 295 kb)

10.6084/m9.figshare.9760775

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Assembly of Metal–Phenolic Networks onto Microbubbles for One‐Step Generation of Functional Microcapsules

Small (2023)

Xin Tan Renwang Sheng Zonghao Liu Weikun Li Renqiang Yuan

Abstract The one‐step assembly of metal–phenolic networks (MPNs) onto particle templates can enable the facile, rapid, and robust construction of hollow microcapsules. However, the required template removal step may affect the refilling of functional species in the hollow interior space or the in situ encapsulation of guest molecules during the formation of the shells. Herein, a simple strategy for the one‐step generation of functional MPNs microcapsules is proposed. This method uses bovine serum albumin microbubbles (BSA MBs) as soft templates and carriers, enabling the efficient pre‐encapsulation of guest species by leveraging the coordination assembly of tannic acid (TA) and Fe III ions. The addition of TA and Fe III induces a change in the protein conformation of BSA MBs and produces semipermeable capsule shells, which allow gas to escape from the MBs without template removal. The MBs‐templated strategy can produce highly biocompatible capsules with controllable structure and size, and it is applicable to produce other MPNs systems like BSA‐TA‐Cu II and BSA‐TA‐Ni II . Finally, those MBs‐templated MPNs capsules can be further functionalized or modified for the loading of magnetic nanoparticles and the pre‐encapsulation of model molecules through covalence or physical adsorption, exhibiting great promise in biomedical applications.

Template

Bovine serum albumin

Tannic acid

10.1002/smll.202305325

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Citations (6)

Durable LBL microcapsule-based sponges with superhydrophobic/super-oleophilic and photothermal behaviors for oil–water separation

Separation and Purification Technology (2023)

Ning Yang Renqiang Yuan Weikun Li Xin Tan Zonghao Liu

Sponge

10.1016/j.seppur.2023.124915

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Citations (12)